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Multipotent embryonic spinal cord stem cells - promote functional recovery after SCI

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    Multipotent embryonic spinal cord stem cells - promote functional recovery after SCI

    Multipotent embryonic spinal cord stem cells expanded by endothelial factors and Shh/RA promote functional recovery after spinal cord injury.

    2007 Oct 12; [Epub ahead of print]

    Lowry N, Goderie SK, Adamo M, Lederman P, Charniga C, Gill J, Silver J, Temple S.

    Center for Neuroscience and Neuropharmacology, Albany Medical Center, Albany NY, USA; New York Neural Stem Cell Institute, Rensselaer, NY, USA.

    Cell transplantation is a promising way to treat spinal cord injury and neurodegenerative disorders. Neural stem cells taken from the embryonic spinal cord are an appealing source of cells for transplantation because these cells are committed to making spinal cord progeny. However these stem cells are rare and require expansion in tissue culture to generate sufficient cells for transplantation. We have developed a novel method for expanding embryonic mouse spinal cord stem cells using a co-culture system with endothelial cells. This method improves neural stem cell survival and preserves their multipotency, including their ability to make motor neurons. Transplantation of endothelial-expanded neural stem cells that were treated with sonic hedgehog(Shh) and retinoic acid (RA) during the expansion phase, into an adult mouse SCI model resulted in significant recovery of sensory and motor function.

    PMID: 18029281 [PubMed - as supplied by publisher]
    What are the differences between neural stem cells taken from the embryonic spinal cord like in this article and neural stem cells, which can be harvested/taken from patients like during some epileptic surgeries, if any differences? Over here some researchers wants to use neural stem cells obtained from the latter technique in SCI studies. Some has also already used them (neural stem cells obtained trough epileptic surgeries) over here in studies like for expansion in tissue culture to generate sufficient cells for transplantation when looking at some brain disorders -They’ve made functional brain cells in the lab that also can communicate with each other.
    Last edited by Leif; 27 Nov 2007, 1:13 PM.

    Also, compared to this it seams like brain-derived neural stem cells (neural stem cells) might be a tad better than neuron-like cells generated from bone marrow-derived progenitors;

    Comparison of neuron-like cells derived from bone marrow stem cells to those differentiated from adult brain neural stem cells.

    2007 Oct

    Song S, Song S, Zhang H, Cuevas J, Sanchez-Ramos J.

    Department of Neurology, University of South Florida, Tampa, FL 33612., James Haley VA Hospital, Tampa, FL 33612.

    Bone marrow-derived stem/progenitor cells have been shown by independent investigators to give rise to neural-like cells (neurons and glia) both in vitro and in vivo. The objective of the present study was to determine whether nestin-enriched cells derived from bone marrow can differentiate into cells with the same morphological and functional characteristics as neurons derived from adult brain neurogenic zones. Cell culture methods were used for generation of adult bone marrow and brain stem/progenitor cells and for studying their differentiation into neural-like cells. The proportion of cells expressing neuronal markers was greater in cultures derived from adult hippocampal neural stem cells than in the bone marrow-derived cells, but the electrophysiological and functional characteristics of the cells were similar. Action potentials with electrical characteristics corresponding to those exhibited by adult neural stem cell-derived neurons were recorded from approximately 2.5% of patched neuron-like cells differentiated from bone marrow cells. The active uptake of tritium-labeled neurotransmitters gamma-aminobutyric acid ([(3)H]GABA) and dopamine ([(3)H]DA) was measured in both sets of cultures. [(3)H]GABA uptake, but not [(3)H]DA, was significantly increased in differentiated neurons in both neural stem cell cultures and bone marrow-derived cultures. [(3)H]GABA uptake was greater in differentiated neurons derived from brain neural stem cells. In summary, both the nestin-expressing bone marrow and the adult brain neural stem/progenitors developed into cells with morphological, immunocytochemical, and functional characteristics of neurons. Even though a smaller proportion of neuron-like cells was generated from bone marrow-derived progenitors than from brain-derived neural stem cells, these cells may be useful in the cellular therapy of neurodegenerative diseases and traumatic brain and spinal cord injury.

    PMID: 17999596 [PubMed - in process]
    One has heard quite a lot of haematopoietic stem cells. But are neural stem cells “underestimated”, or is it just that neural stem cells might be harder to obtain than haematopoietic stem cells?
    Last edited by Leif; 27 Nov 2007, 1:35 PM.