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Transdifferentiated bone marrow mesenchymal cells used, 2 pt chronic SCI in Argentina

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  • Transdifferentiated bone marrow mesenchymal cells used, 2 pt chronic SCI in Argentina

    I will comment later.

    [*] Moviglia G, Fernandez Vina R, Brizuela J, Saslavsky J, Vrsalovic F, Varela G, Bastos F, Farina P, Etchegaray G, Barbieri M, Martinez G, Picasso F, Schmidt Y, Brizuela P, Gaeta C, Costanzo H, Moviglia Brandolino M, Merino S, Pes M, Veloso M, Rugilo C, Tamer I and Shuster G (2006). Combined protocol of cell therapy for chronic spinal cord injury. Report on the electrical and functional recovery of two patients. Cytotherapy 8: 202-9. BackgroundThis is a preliminary report on successful results obtained during treatment of two patients with chronic spinal cord injury. The therapeutic approach was based on the generation of controlled inflammatory activity at the injury site that induced a microenvironment for the subsequent administration of autologous, BM-driven transdifferentiated neural stem cells (NSC).MethodsBM mesenchymal stem cells (MSC) were cocultured with the patient's autoimmune T (AT) cells to be transdifferentiated into NSC. Forty-eight hours prior to NSC implant, patients received an i.v. infusion of 5x108 to 1x109 AT cells. NSC were infused via a feeding artery of the lesion site. Safety evaluations were performed everyday, from the day of the first infusion until 96 h after the second infusion. After treatment, patients started a Vojta and Bobath neurorehabilitation program.ResultsAt present two patients have been treated. Patient 1 was a 19-year-old man who presented paraplegia at the eight thoracic vertebra (T8) with his sensitive level corresponding to his sixth thoracic metamere (T6). He received two AT-NSC treatments and neurorehabilitation for 6 months. At present his motor level corresponds to his first sacral metamere (S1) and his sensitive level to the fourth sacral metamere (S4). Patient 2 was a 21-year-old woman who had a lesion that extended from her third to her fifth cervical vertebrae (C3-C5). Prior to her first therapeutic cycle she had severe quadriplegia and her sensitive level corresponded to her second cervical metamere (C2). After 3 months of treatment her motor and sensitive levels reached her first and second thoracic metameres (T1-T2). No adverse events were detected in either patient.DiscussionThe preliminary results lead us to think that this minimally invasive approach, which has minor adverse events, is effective for the repair of chronic spinal cord lesions. Fundacion Regina Mater, Buenos Aires, Argentina. http://www.ncbi.nlm.nih.gov/entrez/q..._uids=16793729
    [*] Moviglia G, Varela G, Gaeta C, Brizuela J, Bastos F and Saslavsky J (2006). Autoreactive T cells induce in vitro BM mesenchymal stem cell transdifferentiation to neural stem cells. Cytotherapy 8: 196-201. BackgroundThe degree of post-injury inflammation of the damaged area of a spinal cord is the main difference between the natural successful repair in inferior vertebrates and failure in superior vertebrates. The treatment of rats with anti-myelin lymphocytes after experimental spinal cord injury induces their functional recovery. On the other hand, mesenchymal stem cells (MSC) from adult BM implanted in injured areas recover the morphology and function of spinal cord in mammals. The purpose of this study was to determine whether there is a direct relationship between anti-nervous tissue T cells and MSC reparatory properties.MethodsCirculating autoreactive lymphocytes of patients with spinal cord injuries and amyotrophic lateral sclerosis were isolated and activated in vitro. These cells were cocultured with autologous MSC for 2-15 days. Cocultures of non-selected lymphocytes were used as controls.ResultsAfter 48 h of coculture, MSC adopted a spindle shape with polarization of the cytoplasm that resembled bipolar neurons. Their nuclei diminished the nucleolus number and the chromatin lost its granular appearance. After 15 days of culture the cells developed the typical structure of a neural network. No morphologic changes were observed in control cultures. The differentiated cells reacted positively to tubuline III, GFAP and nestin. No differences were observed between the different patient cell sources.DiscussionWe observed that autoreactive cells may induce the transdifferentiation of MSC to neural stem cells. This T-cell-MSC interaction may be a common phenomenon during physiologic nerve tissue repair. Instituto Regina Mater, Buenos Aires, Argentina. http://www.ncbi.nlm.nih.gov/entrez/q..._uids=16793728

  • #2
    Look forward to it Wise.......this sounds HUGE

    Although a small sample this is a scientific FIRST is it not

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    • #3
      Full reports can be found on the Fundacion's site in the Publications section:
      http://www.reginamater.com/

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      • #4
        Fix My Paws

        Funny how interested I get and how closely I can pay attention when the trial yeilds positive results. I would do anything to have strong hands again and while this is only a two person trial the lady went from C3 to T1. Even though there was data I don't understand I am impressed. I am interested to read what Dr. Young has to say.

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        • #5
          C3 to T1, this sounds great. Recovery of my upper body would be a drastic change in my quality of life. Quads dream with something like this.
          -Ramps in buildings are necessary, but it would be usefull to have another ones for people (mind/heart).....

          -Hoc non pereo habebo fortior me

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          • #6
            sounds like a dream comes true. Dr Young what is your opinion?

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            • #7
              "The preliminary results lead us to think that this minimally invasive approach, which has minor adverse events, is effective for the repair of chronic spinal cord lesions."

              Sounds good, sign me up.


              Another aspect of this work is the fact that it is beneficial for chronics AND acutes, since acutes will eventually become chronic, a win-win.
              Last edited by Schmeky; 07-03-2006, 01:55 PM.

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              • #8
                If I may speculate, I think Regina Mater is a Catholic place (based on the name). If so, and this research holds true, then Catholics have done a good bit for us.

                This looks to be a preliminary report. The studies appear to be ongoing.
                ...it's worse than we thought. it turns out the people at the white house are not secret muslims, they're nerds.

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                • #9
                  Im obviously all for scientific and peer reviewed data yet a quick google search on 'regina mater' and I have my concerns

                  por ejemplo....

                  http://216.239.51.104/search?q=cache...&ct=clnk&cd=98

                  Hope I'm wrong

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                  • #10
                    In this Regina Mater was the foundation/Institution which funded the study (Steve have you been to many Latin countries) and it looks like the girl at least will have more treatments. Looks like something has happen on the males MRI’s as well, but are looking forward to comments from Wise. http://www.reginamater.com/ (publications).

                    (Looks like they are advertising for several stem cell treatments, i.e. MS, Parkinson’s and Diabetes).

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                    • #11
                      My mini-write-up:

                      Overview

                      This is a preliminary report, which means further results may be forthcoming.

                      Bone marrow mesenchymal stem cells (MSCs) and autoreactive T cells (ATs) were gathered from two patients. Some ATs were cultured with the MSCs to cause the MSCs to form neural stem cells (NSCs).

                      The patient was then primed. An IV infusion of ATs allegedly recreates the microenvironment of the acute injury, effectively making chronics acute again. 48 hours later, the patients received an infusion of NSCs into an artery near their lesions that supplies the spinal cord with blood.

                      The first, a 19 year old guy para, was (functionally) a T8 when he entered. He plateaued after three months, although the paper fails to say what level he plateaued at. He was given a second round of therapy, and he reached (functionally) S1 by six months. The paper states that he has received a third round and is still working at improving.

                      The second, a 21 year old gal quad, was (functionally) a C3, with some gross hand movement, when she entered. One round later, she was (functionally) a T1-2. She was able to feed herself, brush her teeth, write, paint, and you know the rest.

                      This looks good, but...

                      This place, Regina Mater, has been recruiting patients with a variety of ailments for over two years. How many SCId patients have been treated in total, we do not know. How many recovered, we do not know.

                      However, the science this treatment is based on looks sound, so there's a chance it is true.
                      Leif, I have never been outside of the US.
                      ...it's worse than we thought. it turns out the people at the white house are not secret muslims, they're nerds.

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                      • #12
                        I would like to know what the article means by "he reached (functionally) S1 by six months".

                        I assume "functionally" to mean ambulatory. Perhaps more information is forthcoming in the near future.

                        Returning the chronic lesion to an acute condition through an intravenous injection is a fascinating concept. If this is indeed the case, the difficulty in obtaining human chrondriotinase could be a moot point.

                        Additionally, I would think that with regular scheduled follow-up injections, a quad may be able to gain ambulation. I hope accurate, standardized, neurological assessments were performed before the procedures thus providing a baseline. Look forward to more detailed information.

                        At least this is a chronic therapy which is a more reliable indicator of the effectiveness of emerging therapies, IMO.

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                        • #13
                          Steven, I just meant that crocks can sail under religious flags as well, don’t know if this is the case here tough… let’s see, but there is similarities with other experimental treatments places, offering stem cell treatments for a variety of illnesses and injuries.

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                          • #14
                            Schmeky, the glial scar would still exist. The female patient was at least 28 months post, so I assume she had such a scar. The paper mentioned nothing of removing it, so the point, as you say, may be moot.
                            ...it's worse than we thought. it turns out the people at the white house are not secret muslims, they're nerds.

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                            • #15
                              go team

                              f
                              ight

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