Effect of a polyherbal formulation, Ambrex, on butylated hydroxytoluene (BHT) induced toxicity in rats

Devi, R. S.; Narayan, Shoba; Mohan, K. Vijai; Sabitha, K. E.; Devi, C. S-Shyamala 2003
Indian Journal of Experimental Biology 41(11): 1294-1299

Effect of polyherbal formulation Ambrex was evaluated in butylated hydroxytoluene (BHT) induced toxicity of lungs and liver in rats. Toxicity was produced by administering BHT (500 mg/kg/day) for 3 days. Lung damage was evidenced by elevated levels of broncho alveolar lavage fluid (BAL) parameters such as protein, lactate, lactate dehydrogenase (LDH), alkaline phosphatase (ALP), acid phosphatase (ACP) and glucose-6-phosphate dehydrogenase (G6PDH). Liver damage was proved by elevated levels of serum protein and markers such as LDH, ALP, aspartate amino transferase (AST), alanine amino transferase (ALT), decreased level of lipid peroxides (LPO) in serum and glutathione (GSH) in liver. Administration of aqueous suspension of Ambrex (50 mg/kg orally) retained these elevated levels of BAL-protein, lactate, LDH, ALP, ACP, G6PDH and serum-protein, LDH, ALP, AST and ALT at near normal values. Decreased level of liver GSH was retained at near normalcy in Ambrex pretreated BHT-administered animals. There was no change in liver LPO in all the four groups.

---

Who use BHT:

Most Manufactures of oils, cereals, packaged ready to eat food, Chewing gums mainly use BHT / BHA used as preservative,  may be responsible for some disease condition which occurs to some humans not due to hereditary, since no immediate  effect is not known immediately.